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	Provedor de dados Infoteca-e (1) Autor BRUNE, S. (1) REIFSCHNEIDER, F. J. B. ed. (1) RIBEIRO, C. S. da C. (1) Palavra-chave Acaro do bronzeamento (1) Aculops Lycopersici (1) Agrotis Ipsilon (1) Bemisia argentifolii (1) Berinjela (1) Broca-grande (1) Broca-pequena (1) Burrinho (1) Controle Químico (1) Corythaica cyalthicollis (1) Cropping systems (1) Cultivation (1) Cultivo (1) Dano (1) Diabrotica Speciosa (1) Eggplant (1) Epicauta atomaria (1) Farming systems (1) Frankliniella schultzei (1) Helicoverpa Zea (1) Mais... Tipo do documento Outras publicações técnicas (INFOTECA-E) (1) Ano 1998 (1) País Brazil (1) Idioma Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 174 Primeira ... 1 2 3 4 5 6 7 8 9 ... Última Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil BJM Albano,Ana Paula Neuschrank; Klafke,Gabriel Baracy; Brandolt,Tchana Martinez; Da Hora,Vanusa Pousada; Nogueira,Carlos Eduardo Wayne; Xavier,Melissa Orzechowski; Meireles,Mário Carlos Araújo. Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and... Tipo: Info:eu-repo/semantics/article Palavras-chave: ELISA; Gp-43; Protein G-peroxidase; Paracoccidioidomycosis. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513 Serologic survey for ophidian paramyxovirus in captive snakes MV&Z Paiva, Maria Isabel Sousa; Araújo Junior, João Pessoa; Barraviera, Benedito. The captivity maintenance of snakes is a challenge for the breeders, especially in diseases control. The most common diseases in serpentarium and private collections are the parasites, gastrointestinal problems and respiratory viruses. The biggest concern is the ophidian paramyxovirosis caused by ophidian paramyxovirus (OPMV), which primarily affects the respiratory system and may also involve the nervous system. Often fatal, it was described in Colubridae, Elapidae, Viperidae, Boidae and Pitonidae. This virus is transmitted by aerosols, respiratory secretions, contaminated utensils, ectoparasites such as ticks and digestive excreta. There is no specific treatment or vaccine available. The diagnostic methods are: virus isolation in cell culture; electron... Tipo: Info:eu-repo/semantics/article Palavras-chave: Snakes; Ophidian paramyxovirus; Infection; ELISA; Prophylaxis serpentes; Paramixovirose ofídica; Infecção; ELISA; Profilaxia. Ano: 2016 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31033 Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies in cattle in southern Egypt OAK Fereig, Ragab M.; AbouLaila, Mahmoud Rezk; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, Mosaad; Zaid, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi. Neospora caninum and Cryptosporidium parvum are intracellular protozoan parasites that are distributed worldwide and of major economical concern in cattle industry. N. caninum can cause abortion storms and high culling rates, whereas C. parvum has zoonotic implications and can cause diarrhea in calves. There are currently no data on the prevalence of neosporosis and cryptosporidiosis in humans or animals in southern Egypt. Prevalence of these two infections was determined in a sample of cattle from two different areas in southern Egypt, Sohag and Qena, using enzyme-linked immunosorbent assay. A total 301 cattle were sampled, of which 18.9% were positive for N. caninum, 35.9% were positive for C parvum and 10.0% were positive for both. Geographical location... Palavras-chave: Cattle; Cryptosporidium parvum; Neospora caninum; ELISA; Egypt. Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4531 Serological prevalence and risk factors of Borrelia burgdorferi in water buffaloes (Bubalus bubalis, Linnaeus, 1758) on Marajó Island, northern region of Brazil Rev Salud Anim. Barbosa da Silva,Jenevaldo; Baêta,Bruna de A.; Lopes,Cinthia T.A.; Martins Land Manier,Bruna Sampaio; Nunes Santana de Castro,Gustavo; Nunes dos Santos,Priscilla; da Fonseca,Adivaldo Henrique; Diomedes Barbosa,José. Sera samples were collected from 330 water buffaloes on Marajó Island, state of Pará, Brazil, to assess the presence of antibodies against Borrelia burgdorferi by indirect Enzyme Linked Immunosorbent Assay. Approximately 45% of the animals had antibodies against B. burgdoferi. The prevalence of seropositive buffaloes, 72% (85/118), was statistically higher in the city of Soure than in the other municipalities tested. Murrah breed animals were significantly more seropositive (Prevalence Ratio (PR) = 1.84, p = 0.000) than those of the Mediterranean breed. Among the animals diagnosed positive for tuberculosis, 33% (4/12), were also seropositive for B. burgdoferi. Animals positive for tuberculosis had a significantly lower level of B. burgdorferi... Tipo: Journal article Palavras-chave: Spirochetes; Borrelia sp.; ELISA; Buffaloes; Risk factors; Espiroquetas; Borrelia sp.; ELISA; Búfalos; Factores de riesgo. Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000300002 Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein Arq. Bras. Med. Vet. Zootec. Cândido,A.L.; Resende,M.; Bessa,L.R.G.; Leite,R.C.. A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine herpesvirus-1.2a; Glycoprotein D gene; DNA vaccine; ELISA. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002 SEROLOGICAL SCREENING FOR INFECTIOUS CATTLE DISEASES: II. ASSOCIATION BETWEEN PREVALENCE AND LEVEL OF ELISA RESPONSE Ciência Rural Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles. A herd of cattle (Holstein-Zebu crosses) was screened every two months by ELISA during a period of two years for IgG antibodies against 19 infectious disease agents. Two hundred and ninety five sera were collected from 157 young animals (0-4 months of age), 1037 sera from 292 developing animals (4-36 months of age) and 1468 sera from 259 producing animals (> 36 months of age). The results indicate that the difference in ELISA between positive and negative tests is associated with the overall prevalence of positive tests. When the prevalence of positive tests is low the difference between positive and negative tests is greater than when the prevalence is intermediate or high. This means that ELISA, presumably other serological tests for IgG antibodies,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Serological screening; Cattie diseases; ELISA. Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200014 SEROLOGICAL SCREENING FOR INFECTOUS CATTLE DISEASES: III. CHOICE OF SENTINEL ANIMALS Ciência Rural Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles. A herd of Cattle (Holstein-Zebu crosses) was screened bimonthly for two years by ELISA for IgG antibodies to infectious disease agents. The herd was composed of 3 age groups: Young animals ( < 4 months of age), developing animals (4-36 months of age) and producing animals ( &gt; 36 months of age). Ali young animals received colostrum within two hours after birth. The overall consistency of ELISA results, i. e agreement between two consecutive tests was about the same for the three age groups and was lowest for infections of intermediate prevalence (40-50%). The interpretation of changes in seroprevalence is most straight forward for young animals. Young animals positive at their first test reflect the immune status of their mothers while young... Tipo: Info:eu-repo/semantics/article Palavras-chave: ELISA; Seroconversion; Young; Sentinels; Infectious diseases. Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200015 Serological survey for bovine herpesvirus 1 in cattle from different regions in the state of Bahia, Brazil Braz. J. Vet. Res. Anim. Sci. CERQUEIRA,Robson Bahia; CARMINATI,Renato; SILVA,Josiane Martins; SOARES,Gubio Campos; MEYER,Roberto; SARDI,Silvia. The presence of antibodies against BHV-1 was evaluated in young bovines from fifteen different counties distributed along North, South and Central regions of the state of Bahia. Bovines with age ranged from 1 to 4 years old, non-vaccinated against BHV-1 and without diagnostic of BHV-1 infection were chosen for blood collection. A total of 558 serum samples were collected equivalent to 10% of the population in each farm. The sera were submitted to ELISA (HerdCheck-IDDEX) and Microserum neutralization (MSN) tests. The MSN was performed in monolayers of MDBK cells in microplates using Los Angeles BHV-1 strain. The results of serum samples by ELISA showed that 56% (314/558) were positive and 44% (244/558) were negative. The sera assayed by MSN showed that 48%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine herpesvirus 1 (BHV-1); ELISA; Serumneutralization; Diagnosis. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600014 Serological survey of antibodies to Toxoplasma gondii in food animals from São Paulo state, Brazil Braz. J. Vet. Res. Anim. Sci. Meireles,Luciana Regina; Galisteo Jr.,Andrés Jimenez; Andrade Jr.,Heitor Franco de. Toxoplasmosis is one of the most prevalent parasitic infections of man and livestock, and its transmission has usually been attributed to ingestion of undercooked or raw meat from infected livestock, with the infection rate in those animals being an important risk predictor of human disease, high in Brazil and São Paulo State. Looking for this public health problem, we tested serum samples from cattle, goat, sheep and chicken from the State of São Paulo, Brazil, for IgG antibodies to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA). Antibodies to Toxoplasma gondii were found in 31.00% (62/200) of sheep, 17.00% (34/200) of goat and 11.00% (22/200) of cattle, without positive sample in chicken. Despite differences in feeding habits of each... Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Food animals; Seroprevalence; ELISA. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000400005 Seroprevalence of antibody to NcSAG1 antigen of Neospora caninum in cattle from Western Java, Indonesia OAK ICHIKAWA-SEKI, Madoka; GUSWANTO, Azirwan; ALLAMANDA, Puttik; MARIAMAH, Euis Siti; WIBOWO, Putut Eko; NISHIKAWA, Yoshifumi. Neospora caninum can cause fetal abortion and neonatal mortality in cattle, and is a cause of economic concern worldwide. This study aimed to determine the prevalence of Neospora caninum-specific antibodies in cattle from Western Java, Indonesia. Serum samples from 991 cattle from 21 locations were tested for antibodies to N. caninum by using an enzyme-linked immunosorbent assay (ELISA) on the basis of recombinant NcSAG1. The overall seroprevalence was 16.6%, ranging from 0 to 87.5% in the sampled locations. The results of this study indicate latent infection rates of sampled animals were different in each location. Further studies are necessary to elucidate the relationship between N. caninum infection and abortion in cattle, and to identify risk factors... Palavras-chave: Cattle; ELISA; Indonesia; NcSAG1; Neospora caninum. Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4250 Seroprevalence of Babesia caballi and Theileria equi in horses and mules from Northern Thailand OAK Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X.. Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi. Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092 Seroprevalence of bovine echinococcusis in Pakistan Ciência Rural Aziz,Fariha; Tasawar,Zahida; Lashari,Mushtaq Hussain. ABSTRACT: Cystic echinococcosis is a parasitic infection of worldwide distribution, which, despite causing significant loss of health and money, is still a neglected disease. The present study was performed to determine the prevalence of bovine hydatid disease at Research Centre for the Conservation of Sahiwal Cattle, Jahangirabad, Khanewal, Pakistan. Five hundred bovine serum samples were tested for the detection of bovine Echinococcus antibodies (IgG), using the ELISA kits. Antibodies of bovine Echinococcus were detected in 24.2% cattle. Female cattle showed higher disease prevalence (33.2%) compared to (6.02%) in male cattle. There was positive association between sex of cattle and prevalence (P<0.05). Positive correlation between disease prevalence... Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus antibodies; Seroprevalence; ELISA; Age; Sex. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400501 Seroprevalence of Pythium insidiosum infection in equine in Rio Grande do Sul, Brazil Ciência Rural Weiblen,Carla; Machado,Gustavo; Jesus,Francielli Pantella Kunz de; Santurio,Janio Morais; Zanette,Régis Adriel; Pereira,Daniela Sabel Brayer; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Corbellini,Luis Gustavo; Botton,Sônia de Avila. ABSTRACT: An epidemiological survey was carried out by performing an Enzyme Linked Immuno Sorbent Assay (ELISA) test to determine the seroprevalence of Pythium insidiosum infection in equine in Rio Grande do Sul State (RS), Brazil. The serological study covered seven geographical regions of RS, classified according to the Instituto Brasileiro de Geografia e Estatística (IBGE). The samples were obtained from official veterinary service (Serviço Veterinário Oficial, SVO) linked to the Secretaria da Agricultura, Pecuária e Agronegócio of RS (SEAPA-RS) to proceed the investigation of equine infectious anemia in 2014. Samples were collected during the months of September and October of 2013, covering the seven geographical regions of RS, and totalized 1,002... Tipo: Info:eu-repo/semantics/article Palavras-chave: P. insidiosum; Antibody; Occurrence; Horses; ELISA. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100126 Seroprevalencia de Sarcocystis spp. en un hato lechero del municipio de Toca, Colombia Rev Salud Anim. Pulido-Medellín,Martín O; García-Corredor,Diego; Andrade-Becerra,Roy. Se determinó la seroprevalencia de Sarcocystis spp. en los bovinos de un hato lechero del municipio de Toca, Colombia y su relación con la edad, estado reproductivo y número de partos, utilizando la técnica de ELISA desarrollada por la Fundación Colombiana de Estudios de Parásitos (FUNCEP); la muestra calculada con un nivel de confianza de 95% fue de 100 animales para la realización de la investigación. La prevalencia de Sarcocystis spp. encontrada en este estudio fue del 64%; se observó que el parásito puede presentarse a cualquier edad pero con mayor cantidad de individuos infectados en edades entre los 37 y 48 meses (20.3%). La infección con Sarcocystis spp. puede presentarse en vacas gestantes o vacías sin diferencias entre ellas; sin embargo, se... Tipo: Journal article Palavras-chave: Sarcocystis; ELISA; Seroprevalencia; Bovinos; Parasitología. Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000300003 Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit J. Venom. Anim. Toxins incl. Trop. Dis. Tan,NH; Ponnudurai,G; Fung,SY. The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in serum peaked 12 hours after the injection, followed by a gradual decline and finally reached low rates 72 hours after administration. The serum kinetic profile of venom components, however, did not correspond to the profile of the whole C. rhodostoma venom. The serum levels of the C. rhodostoma thrombin-like enzyme increased slowly and peaked only 48 hours post-injection. Then both thrombin-like enzyme... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calloselasma rhodostoma venom; Thrombin-like enzyme; Hemorrhagin; Serum kinetics; Rabbit; ELISA. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200014 Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases BJMBR Wei,Xinhuan; Yu,Haibin; Zhao,Peng; Xie,Li; Li,Li; Zhang,Jing. Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-ACLF) patients, and 33 healthy controls. The ELISA demonstrated an appropriate linear range, and high levels of reproducibility, sensitivity, specificity,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Regucalcin; Liver injury; Chronic hepatitis B infection; ELISA; Liver failure. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000605 Signal to cut-off (S/CO) ratio and detection of HCV genotype 1 by real-time PCR one-step method: is there any direct relationship? BJID Albertoni,Guilherme; Arnoni,CP; Araújo,PRB; Carvalho,FO; Barreto,JA. BACKGROUND: Polymerase chain reaction (PCR) methods play an essential role in providing data related to diagnosis, monitoring and treatment of hepatitis C virus (HCV) infection. EIA results are reported as ''reactive'' or ''non reactive'' and EIA S/CO ratio may also be reported as ''high'' or ''low.'' This study aimed to evaluate the performance of a real-time RT-PCR and assess whether there is relationship between S/CO and PCR results. STUDY DESIGN AND METHODS: Sera from blood donors were analyzed by Enzyme-Linked Immunosorbent Assay (ELISA) and RT-PCR assay to detect HCV infection. RESULTS: The RT-PCR assay to genotypes 1a/b showed an acceptable linear response in serial dilutions. The samples were divided into two groups based on their serological... Tipo: Info:eu-repo/semantics/article Palavras-chave: HCV; HCV genotype 1; Real-time PCR; ELISA; RNA extraction. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200006 Soroepidemiologia da doença de Newcastle em plantéis de avestruzes dos Estados da Bahia e de São Paulo Ciência Rural Fernandes,Lia Muniz Barretto; Silva,Priscila Sousa da; Ramos,Izabella; Sales,Tatiane Santana; Herval,Elen Fabiane Guimarães; Batinga,Thaís de Brito; Maia,Paulo César Costa; César,André Eduardo Rocha; Doretto Júnior,Luciano; Meyer,Roberto; Freire,Songeli Menezes. Estudos sorológicos em Avestruzes (Struthio camelus) são ferramentas úteis para analisar os riscos relacionados à Doença de Newcastle nesses plantéis e à avicultura nacional. No presente estudo, amostras de sangue foram obtidas de avestruzes de ambos os sexos, de diferentes faixas etárias e sem apresentação de sintomatologia clínica, criadas nos Estados da Bahia e de São Paulo com o objetivo de avaliar a presença de anticorpos contra o vírus da Doença de Newcastle por meio de ELISA indireto. Foram testadas 339 amostras provenientes do Estado da Bahia e 105 amostras do Estado de São Paulo. Apesar de os proprietários afirmarem que não foi utilizada vacina em seus animais, foi verificada positividade na Bahia de 17,9% e de 4,7% em São Paulo, em avestruzes,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Avestruz; Doença de Newcastle; ELISA; Diagnóstico. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000100022 Stabilising selection on immune response in male black grouse Lyrurus tetrix ArchiMer Soulsbury, Carl D.; Siitari, Heli; Lebigre, Christophe. Illnesses caused by a variety of micro- and macro- organisms can negatively affect individuals' fitness, leading to the expectation that immunity is under positive selection. However, immune responses are costly and individuals must trade-off their immune response with other fitness components (e.g. survival or reproductive success) meaning that individuals with intermediate response may have the greatest overall fitness. Such a process might be particularly acute in species with strong sexual selection because the condition-dependence of male secondary sexual-traits might lead to striking phenotypic differences amongst males of different immune response levels. We tested whether there is selection on immune response by survival and reproduction in... Tipo: Text Palavras-chave: Ecological immunology; ELISA; Immunocompetance; Life history theory; Stabilising selection. Ano: 2018 URL: http://archimer.ifremer.fr/doc/00426/53761/54653.pdf Summary and Recommendations of the International Workshop on Aflatoxin Contamination of Groundnut Open Agri Palavras-chave: Mycotoxins; Groundnuts; ELISA; Genotypes; Sampling; Detoxification; Chromatography; Toxins; Fungi; Immunological techniques. Ano: 1988 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4056 Registros recuperados: 174 Primeira ... 1 2 3 4 5 6 7 8 9 ... Última

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